MICAL2 antibody

• Background :

  Nuclear monooxygenase that promotes depolymerization of F-actin by mediating oxidation of specific methionine residues on actin and regulates the SRF signaling. Acts by modifying nuclear actin subunits through the addition of oxygen to form methionine-sulfoxide, leading to promote actin filament severing and prevent repolymerization. Acts as a key regulator of the SRF signaling pathway elicited by nerve growth factor and serum: mediates oxidation and subsequent depolymerization of nuclear actin, leading to increase MKL1/MRTF-A presence in the nucleus and promote SRF:MKL1/MRTF-A-dependent gene transcription. Does not activate SRF:MKL1/MRTF-A through RhoA.

• Synonyms :

  [F-actin]-monooxygenase MICAL2|MICAL C-terminal-like protein (Mical-cL) |Molecule interacting with CasL protein 2 (MICAL-2) |MICAL2|KIAA0750|MICAL2PV1|MICAL2PV2|MICALCL

• Gene ID :

  9645

• UniProt :

  O94851

• Host :

  Rabbit

• Reactivity :

  Human, Mouse, Rat

• Immunogen :

  Microtubule associated monoxygenase, calponin and LIM domain containing 2

• Target :

  MICAL2

• Clonality :

  Polyclonal

• Isotype :

  IgG

• Applications :

  ELISA, IHC, IF

• Field of Research :

  Metabolism, Developmental biology

• Purification :

  Immunogen affinity purified

• Dilution :

  IHC: 1:20-1:200; IF: 1:20-1:200

• Purity :

  ≥95% as determined by SDS-PAGE

• Form :

  Liquid

• Molecular Weight :

  95, 112 kDa

• Shipping Conditions :

  4°C with ice bag

• Storage Conditions :

  PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

• Tested Applications :

  ELISA, IHC, IF