MBP tag antibody

MBP tag antibody

• Background: Protein tags are protein or peptide sequences located either on the C-or N-terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Maltose binding protein(MBP) is the 370 amino acid product of the E.coli mal E gene. MBP is a useful affinity tag that can increase the expression level and solubility of the resulting tagged protein. The MBP tag also promotes proper folding of the attached protein. Plasmid vectors have been constructed utilizing the MBP domain that allow the synthesis of high levels of MBP-fusion proteins that can be purified in a one step procedure by affinity chromatography cross linked amylose resin.
• Synonyms: Maltose Binding Protein|MBP|MBP Tag
• Host: Mouse
• Reactivity: Recombinant protein
• Immunogen: MBP-Tag
• Target: MBP tag
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone: 0D8
• Applications: ELISA, WB, IP
• Purification: Protein A+G purification
• Dilution: WB: 1:1000-1:8000; IP: 1:5000-1:50000
• Purity: ≥95% as determined by SDS-PAGE
• Form: Liquid
• Molecular Weight: 40 kDa
• Storage Conditions: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months(Avoid repeated freeze / thaw cycles.)