LIN28B antibody

• Background :

  Suppressor of microRNA (miRNA) biogenesis, including that of let-7 and possibly of miR107, miR-143 and miR-200c. Binds primary let-7 transcripts (pri-let-7), including pri-let-7g and pri-let-7a-1, and sequester them in the nucleolus, away from the microprocessor complex, hence preventing their processing into mature miRNA (PubMed:22118463) . Does not act on pri-miR21 (PubMed:22118463) . The repression of let-7 expression is required for normal development and contributes to maintain the pluripotent state of embryonic stem cells by preventing let-7-mediated differentiation. When overexpressed, recruits ZCCHC11/TUT4 uridylyltransferase to pre-let-7 transcripts, leading to their terminal uridylation and degradation (PubMed:19703396) . This activity might not be relevant in vivo, as LIN28B-mediated inhibition of let-7 miRNA maturation appears to be ZCCHC11-independent (PubMed:22118463) . Interaction with target pre-miRNAs occurs via an 5'-GGAG-3' motif in the pre-miRNA terminal loop. Mediates MYC-induced let-7 repression (By similarity) . When overexpressed, isoform 1 stimulates growth of the breast adenocarcinoma cell line MCF-7. Isoform 2 has no effect on cell growth.

• Synonyms :

  Protein lin-28 homolog B (Lin-28B) |LIN28B|CSDD2

• Gene ID :

  389421

• UniProt :

  Q6ZN17

• Host :

  Rabbit

• Reactivity :

  Human, Mouse

• Immunogen :

  Lin-28 homolog B

• Target :

  LIN28B

• Clonality :

  Polyclonal

• Isotype :

  IgG

• Applications :

  ELISA, WB

• Field of Research :

  Epigenetics, Stem Cells, Metabolism

• Purification :

  Immunogen affinity purified

• Dilution :

  WB: 1:500-1:2000

• Purity :

  ≥95% as determined by SDS-PAGE

• Form :

  Liquid

• Molecular Weight :

  27 kDa

• Shipping Conditions :

  4°C with ice bag

• Storage Conditions :

  PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

• Tested Applications :

  ELISA, WB

• Immunogen Sequence :

  5-250AA