LIG4 antibody

• Background :

  Efficiently joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V (D) J recombination. The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends.

• Synonyms :

  DNA ligase 4|DNA ligase IV|Polydeoxyribonucleotide synthase [ATP] 4|LIG4

• Gene ID :

  3981

• UniProt :

  P49917

• Host :

  Rabbit

• Reactivity :

  Human, Mouse

• Immunogen :

  Ligase IV, DNA, ATP-dependent

• Target :

  LIG4

• Clonality :

  Polyclonal

• Isotype :

  IgG

• Applications :

  ELISA, WB, IHC

• Field of Research :

  Metabolism

• Purification :

  Immunogen affinity purified

• Dilution :

  WB: 1:500-1:2000; IHC: 1:20-1:200

• Purity :

  ≥95% as determined by SDS-PAGE

• Form :

  Liquid

• Molecular Weight :

  100-104 kDa

• Shipping Conditions :

  4°C with ice bag

• Storage Conditions :

  PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

• Tested Applications :

  ELISA, WB, IHC