HMGB2 antibody

Multifunctional protein with various roles in different cellular compartments. May act in a redox sensitive manner. In the nucleus is an abundant chromatin-associated non-histone protein involved in transcription, chromatin remodeling and V (D) J recombination and probably other processes. Binds DNA with a preference to non-canonical DNA structures such as single-stranded DNA. Can bent DNA and enhance DNA flexibility by looping thus providing a mechanism to promote activities on various gene promoters by enhancing transcription factor binding and/or bringing distant regulatory sequences into close proximity (PubMed:7797075, PubMed:11909973, PubMed:19522541, PubMed:18413230, PubMed:19965638, PubMed:20123072) . Involved in V (D) J recombination by acting as a cofactor of the RAG complex: acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS) (By similarity) . Proposed to be involved in the innate immune response to nucleic acids by acting as a promiscuous immunogenic DNA/RNA sensor which cooperates with subsequent discriminative sensing by specific pattern recognition receptors (By similarity) . In the extracellular compartment acts as a chemokine. Promotes proliferation and migration of endothelial cells implicating AGER/RAGE (PubMed:19811285) . Has antimicrobial activity in gastrointestinal epithelial tissues (PubMed:23877675) . Involved in inflammatory response to antigenic stimulus coupled with proinflammatory activity (By similarity) . Involved in modulation of neurogenesis probably by regulation of neural stem proliferation (By similarity) . Involved in articular cartilage surface maintenance implicating LEF1 and the Wnt/beta-catenin pathway (By similarity) .

High mobility group protein B2|High mobility group protein 2 (HMG-2) |HMGB2|HMG2

3148

P26583

Human, Mouse, Rat

High-mobility group box 2

HMGB2

ELISA, WB, IHC, IP

Metabolism, Developmental biology

Immunogen affinity purified

WB: 1:500-1:2000; IP: 1:200-1:1000; IHC: 1:20-1:200

≥95% as determined by SDS-PAGE

Liquid

33-35 kDa

4°C with ice bag

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)