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PPP1R15A antibody

CAT: 0710-FNab03293Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0710-FNab03293Size:100 µg
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Background
GADD34, also named PPP1R15A, belongs to the PPP1R15 family. GADD34 can be triggered as a direct target of activating transcription factor4 (ATF4) under ER stress, it plays a pivotal role in the recovery of cells from shut-down of translation induced by ER stress. It recruits the serine/threonine-protein phosphatase (PP1) to dephosphorylate the translation initiation factor eIF2alpha, thereby reversing the shut-off of protein synthesis initiated by stress-inducible kinases and facilitating recovery of cells from stress. GADD34 down-regulates the TGF-beta signaling pathway by promoting dephosphorylation of TGFB1 via PP1. It may also promote apoptosis by inducing TP53 phosphorylation on 'Ser-15'. Starvation-induced expression of GADD34 reduced mTOR activity and induced autophagy in wild-type mice, but not in GADD34 KO mice. Molecular weight of GADD34 appears to be about 100 kDa which was confirmed in GADD34 KO mice and drug treated cells, despite the predicated MW of 73 kDa, and this antibody primarily recongize the 100 kDa band.
Synonyms
Protein phosphatase 1 regulatory subunit 15A|Growth arrest and DNA damage-inducible protein GADD34|Myeloid differentiation primary response protein MyD116 homolog|PPP1R15A|GADD34
UniProt
O75807
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Protein phosphatase 1, regulatory (inhibitor) subunit 15A
Target
PPP1R15A
Clonality
Polyclonal
Isotype
IgG
Applications
ELISA, WB, IHC, IF
Field of Research
Epigenetics
Purification
Immunogen affinity purified
Dilution
WB: 1:200-1:1000; IHC: 1:20-1:200; IF: 1:20-1:200
Purity
≥95% as determined by SDS-PAGE
Form
Liquid
Molecular Weight
100 kDa
Shipping Conditions
4°C with ice bag
Storage Conditions
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)
Tested Applications
ELISA, WB, IHC, IF

UniProtKB · O75807

Protein phosphatase 1 regulatory subunit 15A

PR15A_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
O75807
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
PPP1R15A
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
B4DKQ3, Q6IA96, Q9NVU6
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Biological process

ApoptosisStress responseTranslation regulation

Cellular component

Endoplasmic reticulumMembraneMitochondrionMitochondrion outer membrane

PTM

PhosphoproteinUbl conjugation

Domain

Repeat

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