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Flag tag antibody

CAT: 0710-FNab03155Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0710-FNab03155Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Protein tags are protein or peptide sequences located either on the C-or N-terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The DYKDDDDK (FLAG) peptide has been used extensively as a general tag in expression vectors. This antibody is generated against 1xDDDDK tag (DYKDDDDK) and can recognize protein containing one or more DDDDK tags, independently on N-terminal, C-terminal or internal regions of the target protein.
Host
Mouse
Reactivity
Recombinant protein
Immunogen
Flag tag
Target
Flag tag
Clonality
Monoclonal
Isotype
IgG1
Applications
ELISA, WB, IP
Purification
Protein A+G purification
Dilution
WB: 1:500-1:2000; IP: 1:500-1:2000
Purity
≥95% as determined by SDS-PAGE
Form
Liquid
Shipping Conditions
4°C with ice bag
Storage Conditions
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)
Clone ID
2D10
Tested Applications
ELISA, WB, IP
Immunogen Sequence
Peptide

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