Flag tag antibody

• Background:

  Protein tags are protein or peptide sequences located either on the C-or N-terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The DYKDDDDK (FLAG) peptide has been used extensively as a general tag in expression vectors. This antibody is a rabbit polyclonal antibody raised against 3xFlag (3xDYKDDDDKT) sequence and recognizes the (3x) DYKDDDDK peptide and detects FLAG-tagged proteins.

• Host:

  Rabbit

• Reactivity:

  Recombinant protein

• Immunogen:

  Flag tag

• Target:

  Flag tag

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  ELISA, WB, IF

• Purification:

  Immunogen affinity purified

• Dilution:

  WB: 1:1000-1:4000; IF: 1:10-1:100

• Purity:

  ≥95% as determined by SDS-PAGE

• Form:

  Liquid

• Shipping Conditions:

  4°C with ice bag

• Storage Conditions:

  PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

• Tested Applications:

  ELISA, WB, IF