Eif6 antibody

Binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit to form the 80S initiation complex in the cytoplasm. Behaves as a stimulatory translation initiation factor downstream insulin/growth factors. Is also involved in ribosome biogenesis. Associates with pre-60S subunits in the nucleus and is involved in its nuclear export. Cytoplasmic release of TIF6 from 60S subunits and nuclear relocalization is promoted by a RACK1 (RACK1) -dependent protein kinase C activity. In tissues responsive to insulin, controls fatty acid synthesis and glycolysis by exerting translational control of adipogenic transcription factors such as CEBPB, CEBPD and ATF4 that have G/C rich or uORF in their 5'UTR (PubMed:26383020) . Required for ROS-dependent megakaryocyte maturation and platelets formation, controls the expression of mitochondrial respiratory chain genes involved in reactive oxygen species (ROS) synthesis (PubMed:26391622) . Involved in miRNA-mediated gene silencing by the RNA-induced silencing complex (RISC) . Required for both miRNA-mediated translational repression and miRNA-mediated cleavage of complementary mRNAs by RISC (By similarity) . Modulates cell cycle progression and global translation of pre-B cells, its activation seems to be rate-limiting in tumorigenesis and tumor growth (PubMed:21665150) .

Eukaryotic translation initiation factor 6 (eIF-6) |B4 integrin interactor|CAB|p27 (BBP) |Eif6|Itgb4bp

16418

O55135

Human, Mouse, Rat

Eukaryotic translation initiation factor 6

Eif6

ELISA, WB, IHC

Metabolism

Immunogen affinity purified

WB: 1:500-1:2000; IHC: 1:20-1:200

≥95% as determined by SDS-PAGE

Liquid

27-30 kDa

4°C with ice bag

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)