Eif6 antibody

Eif6 antibody

• Background: Binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit to form the 80S initiation complex in the cytoplasm. Behaves as a stimulatory translation initiation factor downstream insulin/growth factors. Is also involved in ribosome biogenesis. Associates with pre-60S subunits in the nucleus and is involved in its nuclear export. Cytoplasmic release of TIF6 from 60S subunits and nuclear relocalization is promoted by a RACK1(RACK1)-dependent protein kinase C activity. In tissues responsive to insulin, controls fatty acid synthesis and glycolysis by exerting translational control of adipogenic transcription factors such as CEBPB, CEBPD and ATF4 that have G/C rich or uORF in their 5'UTR(PubMed:26383020). Required for ROS-dependent megakaryocyte maturation and platelets formation, controls the expression of mitochondrial respiratory chain genes involved in reactive oxygen species(ROS) synthesis(PubMed:26391622). Involved in miRNA-mediated gene silencing by the RNA-induced silencing complex(RISC). Required for both miRNA-mediated translational repression and miRNA-mediated cleavage of complementary mRNAs by RISC(By similarity). Modulates cell cycle progression and global translation of pre-B cells, its activation seems to be rate-limiting in tumorigenesis and tumor growth(PubMed:21665150).
• Synonyms: Eukaryotic translation initiation factor 6 (eIF-6)|B4 integrin interactor|CAB|p27(BBP)|Eif6|Itgb4bp
• Gene ID: 16418
• UniProt: O55135
• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Immunogen: eukaryotic translation initiation factor 6
• Target: Eif6
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ELISA, WB, IHC
• Field of Research: Metabolism
• Purification: Immunogen affinity purified
• Dilution: WB: 1:500-1:2000; IHC: 1:20-1:200
• Purity: ≥95% as determined by SDS-PAGE
• Form: Liquid
• Molecular Weight: 27-30 kDa
• Storage Conditions: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months(Avoid repeated freeze / thaw cycles.)