DNMT1 antibody

• Background:

  Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells (PubMed:24623306) . Also required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs) (PubMed:24623306) . Associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing (PubMed:24623306) . Promotes tumor growth (PubMed:24623306) .

• Synonyms:

  DNA (cytosine-5) -methyltransferase 1 (Dnmt1) |CXXC-type zinc finger protein 9|DNA methyltransferase HsaI (DNA MTase HsaI, M.HsaI) |MCMT|DNMT1|AIM|CXXC9|DNMT

• Gene ID:

  1786

• UniProt:

  P26358

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  DNA (cytosine-5-) -methyltransferase 1

• Target:

  DNMT1

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  ELISA, IHC, IP, WB, IF

• Field of Research:

  Epigenetics, Metabolism

• Purification:

  Immunogen affinity purified

• Dilution:

  WB: 1:500-1:2000; IP: 1:500-1:2000; IHC: 1:20-1:200; IF: 1:10-1:100

• Purity:

  ≥95% as determined by SDS-PAGE

• Form:

  Liquid

• Molecular Weight:

  180-200 kDa

• Shipping Conditions:

  4°C with ice bag

• Storage Conditions:

  PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

• Tested Applications:

  ELISA, IHC, IP, WB, IF