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DGCR8 antibody

CAT: 0710-FNab02353Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0710-FNab02353Size:100 µg
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Background
Component of the microprocessor complex that acts as a RNA-and heme-binding protein that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DGCR8 function as a molecular anchor necessary for the recognition of pri-miRNA at dsRNA-ssRNA junction and directs DROSHA to cleave 11 bp away form the junction to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs (PubMed:26027739, PubMed:26748718) . The heme-bound DGCR8 dimer binds pri-miRNAs as a cooperative trimer (of dimers) and is active in triggering pri-miRNA cleavage, whereas the heme-free DGCR8 monomer binds pri-miRNAs as a dimer and is much less active. Both double-stranded and single-stranded regions of a pri-miRNA are required for its binding (PubMed:15531877, PubMed:15574589, PubMed:15589161, PubMed:16751099, PubMed:16906129, PubMed:16963499, PubMed:17159994) . Specifically recognizes and binds N6-methyladenosine (m6A) -containing pri-miRNAs, a modification required for pri-miRNAs processing (PubMed:25799998) . Involved in the silencing of embryonic stem cell self-renewal (By similarity) .
Synonyms
Microprocessor complex subunit DGCR8|DiGeorge syndrome critical region 8|DGCR8|C22orf12|DGCRK6
Gene ID
54487
UniProt
Q8WYQ5
Host
Mouse
Reactivity
Human, Mouse
Immunogen
DiGeorge syndrome critical region gene 8
Target
DGCR8
Clonality
Monoclonal
Isotype
IgG1
Applications
ELISA, WB, IP
Field of Research
Epigenetics
Purification
Protein A+G purification
Dilution
WB: 1:500-1:2000; IP: 1:500-1:1000
Purity
≥95% as determined by SDS-PAGE
Form
Liquid
Molecular Weight
120 kDa
Shipping Conditions
4°C with ice bag
Storage Conditions
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)
Clone ID
2G8
Tested Applications
ELISA, WB, IP
Immunogen Sequence
430-773aa

UniProtKB · Q8WYQ5

Microprocessor complex subunit DGCR8

DGCR8_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q8WYQ5
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
DGCR8
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
B2R8G1, Q6DCB2, Q6MZE9, Q6Y2L0, Q96G39, Q96GP8, Q9H6L8, Q9H6T7, Q9NRW2
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Ligand

HemeIronMetal-binding

PTM

Isopeptide bondPhosphoproteinUbl conjugation

Cellular component

Nucleus

Domain

Repeat

Molecular function

RNA-binding

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