DDB1 antibody

• Background:

  Required for DNA repair. Binds to DDB2 to form the UV-damaged DNA-binding protein complex (the UV-DDB complex) . The UV-DDB complex may recognize UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers (CPD), 6-4 photoproducts (6-4 PP), apurinic sites and short mismatches. Also appears to function as a component of numerous distinct DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the DCX E3 ubiquitin-protein ligase complex is determined by the variable substrate recognition component recruited by DDB1. DCX (DDB2) (also known as DDB1-CUL4-ROC1, CUL4-DDB-ROC1 and CUL4-DDB-RBX1) may ubiquitinate histone H2A, histone H3 and histone H4 at sites of UV-induced DNA damage. The ubiquitination of histones may facilitate their removal from the nucleosome and promote subsequent DNA repair. DCX (DDB2) also ubiquitinates XPC, which may enhance DNA-binding by XPC and promote NER. DCX (DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of TP53 in response to radiation-induced DNA damage and during DNA replication. DCX (ERCC8) (the CSA complex) plays a role in transcription-coupled repair (TCR) . May also play a role in ubiquitination of CDKN1B/p27kip when associated with CUL4 and SKP2.

• Synonyms:

  DNA damage-binding protein 1|DDB p127 subunit|DNA damage-binding protein a (DDBa) |Damage-specific DNA-binding protein 1|HBV X-associated protein 1 (XAP-1) |UV-damaged DNA-binding factor|UV-damaged DNA-binding protein 1 (UV-DDB 1) |XPE-binding factor (XPE-BF) |Xeroderma pigmentosum group E-complementing protein (XPCe) |DDB1|XAP1

• Gene ID:

  1642

• UniProt:

  Q16531

• Host:

  Mouse

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Damage-specific DNA binding protein 1, 127kDa

• Target:

  DDB1

• Clonality:

  Monoclonal

• Isotype:

  IgG2a

• Applications:

  ELISA, WB, IHC, IF

• Field of Research:

  Epigenetics, Metabolism

• Purification:

  Protein A+G purification

• Dilution:

  WB: 1:500-1:2000; IHC: 1:200-1:800

• Purity:

  ≥95% as determined by SDS-PAGE

• Form:

  Liquid

• Molecular Weight:

  127 kDa

• Shipping Conditions:

  4°C with ice bag

• Storage Conditions:

  PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

• Clone ID:

  3A0

• Tested Applications:

  ELISA, WB, IHC, IF

• Immunogen Sequence:

  1-400aa