CDC25B antibody

• Background:

  CDC25B, also named as CDC25HU2, belongs to the MPI phosphatase family. It is a tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. CDC25B directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity. In Jurkat cell, this antibody recognized 2 bands: 60kd (isoform 2, CDC25B2) and 70kd (isoform 4 of CDC25B) .

• Synonyms:

  M-phase inducer phosphatase 2|Dual specificity phosphatase Cdc25B|CDC25B|CDC25HU2

• Gene ID:

  994

• UniProt:

  P30305

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Immunogen:

  Cell division cycle 25 homolog B (S. pombe)

• Target:

  CDC25B

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  ELISA, WB, IHC, IP

• Field of Research:

  Cancer, Cell Division and Proliferation, Metabolism

• Purification:

  Immunogen affinity purified

• Dilution:

  WB: 1:200-1:1000; IP: 1:200-1:1000; IHC: 1:20-1:200

• Purity:

  ≥95% as determined by SDS-PAGE

• Form:

  Liquid

• Molecular Weight:

  60-70 kDa

• Shipping Conditions:

  4°C with ice bag

• Storage Conditions:

  PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

• Tested Applications:

  ELISA, WB, IHC, IP