CD22 antibody

• Background:

  Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules.

• Synonyms:

  B-cell receptor CD22|B-lymphocyte cell adhesion molecule (BL-CAM) |Sialic acid-binding Ig-like lectin 2 (Siglec-2) |T-cell surface antigen Leu-14|CD22|SIGLEC2

• Gene ID:

  933

• UniProt:

  P20273

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  CD22 molecule

• Target:

  CD22

• Clonality:

  Monoclonal

• Isotype:

  IgG1

• Applications:

  ELISA, WB, IHC

• Field of Research:

  Stem Cells, Immunology, Signal Transduction, Cancer

• Purification:

  Protein A+G purification

• Dilution:

  WB : 1:1000-1:10000; IHC : 1:500-1:2000

• Purity:

  ≥95% as determined by SDS-PAGE

• Form:

  Liquid

• Molecular Weight:

  135 kDa

• Shipping Conditions:

  4°C with ice bag

• Storage Conditions:

  PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

• Clone ID:

  7D10

• Tested Applications:

  ELISA, WB, IHC

• Immunogen Sequence:

  340-687aa