BRAF antibody

• Background:

  This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene.

• Synonyms:

  Serine/threonine-protein kinase B-raf|Proto-oncogene B-Raf|p94|v-Raf murine sarcoma viral oncogene homolog B1|BRAF|BRAF1|RAFB1

• Gene ID:

  673

• UniProt:

  P15056

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  V-raf murine sarcoma viral oncogene homolog B1

• Target:

  BRAF

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  ELISA, WB, IHC

• Field of Research:

  Cancer, Cell Division and Proliferation, Cardiovascular, Signal Transduction

• Purification:

  Immunogen affinity purified

• Dilution:

  WB: 1:500 - 1:2000; IHC: 1:50 - 1:200

• Purity:

  ≥95% as determined by SDS-PAGE

• Form:

  Liquid

• Molecular Weight:

  98 kDa

• Shipping Conditions:

  4°C with ice bag

• Storage Conditions:

  PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

• Tested Applications:

  ELISA, WB, IHC

• Immunogen Sequence:

  8-170aa