APAF1 antibody

• Background:

  Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis.

• Synonyms:

  Apoptotic protease-activating factor 1 (APAF-1) |APAF1|KIAA0413

• Gene ID:

  317

• UniProt:

  O14727

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Apoptotic peptidase activating factor 1

• Target:

  APAF1

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  ELISA, WB, IHC, IP, IF

• Field of Research:

  Developmental biology

• Purification:

  Immunogen affinity purified

• Dilution:

  WB: 1:500-1:2000; IP: 1:500-1:1000; IHC: 1:50-1:500; IF: 1:20-1:200

• Purity:

  ≥95% as determined by SDS-PAGE

• Form:

  Liquid

• Molecular Weight:

  140 kDa

• Shipping Conditions:

  4°C with ice bag

• Storage Conditions:

  PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

• Tested Applications:

  ELISA, WB, IHC, IP, IF