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Recombinant Human CXCL16

CAT: 0355-AP60292Size: InquireDry Ice: NoHazardous: No
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CAT#:0355-AP60292Size:Inquire
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
CXC chemokine ligand 16 (CXCL16) is a type I membrane protein containing a non-ELR motif-containing CXC chemokine domain in its extracellular region. Together with Fractalkine (CX3CL1), CXCL16 constitute the only two transmembrane chemokines within the superfamily. The gene for human CXCL16 predicts a 273 amino acid (aa) residue precursor protein with a putative signal peptide, a CXC chemokine domain, a mucin-like spacer region, a transmembrane domain and a cytoplasmic domain with a potential tyrosine phosphorylation and SH2 protein-binding site. Mouse and human CXCL16 share 70% aa sequence similarity within their chemokine domains and 49% overall aa sequence identity.
Target
Asn49-Pro137; Accession # NP_071342
Purification
> 97 % by SDS-PAGE analyses.
Endotoxin
Less than 1.0 EU/ug of rHuCXCL16 as determined by LAL method.
Bioactivity
Measured by its ability to chemoattract BaF3 mouse pro-B cells transfected with mouse CXCR6. The ED50 for this effect is 2.5-12 ng/ml.
Storage Conditions
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculated Molecular Weight
Approximately 10 kDa, a single non-glycosylated polypeptide chain containing 89 amino acids.
Formulation
Lyophilized from 0.2 μm filtered concentrated solution in PBS.
Host or Source
Escherichia coli

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