SingleFlowEx CD45 PerCP

• Description:

  SingleFlowEx is fluorochrome-labeled monoclonal antibody single reagent for use in laboratory-developed multicolor flow cytometry tests in order to examine human clinical samples. SingleFlowEx can be used in various multicolor combinations. Laboratory shall establish the SingleFlowEx performance characteristics in conjunction with other reagents in normal and pathological samples.

• Certification:

  RUO (GMP)

• Clone:

  MEM-28

• Applications:

  FC (QC tested)

• Concentration:

  0.060 mg/mL

• Format:

  PerCP

• Buffer:

  Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

• References & Citations:

  *Leukocyte Typing III., McMichael A. J. et al (Eds.), Oxford University Press (1987) ., URL: , *Horejsi V, Angelisova P, Bazil V, Kristofova H, Stoyanov S, Stefanova I, Hausner P, Vosecky M, Hilgert I: Monoclonal antibodies against human leucocyte antigens. II. Antibodies against CD45 (T200), CD3 (T3), CD43, CD10 (CALLA), transferrin receptor (T9), a novel broadly expressed 18-kDa antigen (MEM-43) and a novel antigen of restricted expression (MEM-74) . Folia Biol (Praha) . 1988;34 (1) :23-34., URL: https://www.ncbi.nlm.nih.gov/pubmed/2968928?dopt=A, *Ilangumaran S, Briol A, Hoessli DC: CD44 selectively associates with active Src family protein tyrosine kinases Lck and Fyn in glycosphingolipid-rich plasma membrane domains of human peripheral blood lymphocytes. Blood. 1998 May 15;91 (10) :3901-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/9573028?itool=, *Drbal K, Moertelmaier M, Holzhauser C, Muhammad A, Fuertbauer E, Howorka S, Hinterberger M, Stockinger H, Schütz GJ: Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement. Int Immunol. 2007 May;19 (5) :675-84., URL: https://www.ncbi.nlm.nih.gov/pubmed/17446208?itool, *Koethe S, Zander L, Köster S, Annan A, Ebenfelt A, Spencer J, Bemark M: Pivotal advance: CD45RB glycosylation is specifically regulated during human peripheral B cell differentiation. J Leukoc Biol. 2011 Jul;90 (1) :5-19., URL: http://www.ncbi.nlm.nih.gov/pubmed?term=koethe%20s%20zander%20l , *Cermák L, Símová S, Pintzas A, Horejsí V, Andera L: Molecular mechanisms involved in CD43-mediated apoptosis of TF-1 cells. Roles of transcription Daxx expression, and adhesion molecules. J Biol Chem. 2002 Mar 8;277 (10) :7955-61., URL: http://www.ncbi.nlm.nih.gov/pubmed?term=%C4%8Derm%C3%A1k%20l%20%C5%A1%C3%ADmov%C3%A1%20%C5%A1%20pintzas%20a , *McCormack E, Mujić M, Osdal T, Bruserud Ø , Gjertsen BT: Multiplexed mAbs: a new strategy in preclinical time-domain imaging of acute myeloid leukemia. Blood. 2013 Feb 14;121 (7) :e34-42. doi: 10.1182/blood-2012-05-429555., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=osdal+t+bruserud+o , *Majer F, Vlaskova H, Krol L, Kalina T, Kubanek M, Stolnaya L, Dvorakova L, Elleder M, Sikora J: Danon disease: a focus on processing of the novel LAMP2 mutation and comments on the beneficial use of peripheral white blood cells in the diagnosis of LAMP2 deficiency. Gene. 2012 May 1;498 (2) :183-95., URL: https://pubmed.ncbi.nlm.nih.gov/22365987/, *Stranavova L, Pelak O, Svaton M, Hruba P, Fronkova E, Slavcev A, Osickova K, Maluskova J, Hubacek P, Fronek J, Reinke P, Volk HD, Kalina T, Viklicky O: Heterologous cytomegalovirus and allo-reactivity by shared T cell receptor repertoire in kidney transplantation. Frontiers in Immunology 2019, 10:2549, URL:

• Other References:

  *Li FJ, Tsuyama N, Ishikawa H, Obata M, Abroun S, Liu S, Otsuyama K, Zheng X, Ma Z, Maki Y, Kawano MM: A rapid translocation of CD45RO but not CD45RA to lipid rafts in IL-6-induced proliferation in myeloma. Blood. 2005 Apr 15;105 (8) :3295-302. , URL: https://www.ncbi.nlm.nih.gov/pubmed/15626731?ordin, *Cosenza-Nashat MA, Kim MO, Zhao ML, Suh HS, Lee SC: CD45 isoform expression in microglia and inflammatory cells in HIV-1 encephalitis. Brain Pathol. 2006 Oct;16 (4) :256-65., URL: http://www.ncbi.nlm.nih.gov/pubmed/17107594?ordina, *Dawes R, Petrova S, Liu Z, Wraith D, Beverley PC, Tchilian EZ. Combinations of CD45 isoforms are crucial for immune function and disease. J Immunol. 2006 Mar 15;176 (6) :3417-25., URL: http://www.ncbi.nlm.nih.gov/pubmed/16517710?ordina, *Bijian K, Zhang L, Shen SH: Collagen-mediated survival signaling is modulated by CD45 in Jurkat T cells. Mol Immunol. 2007 Jul;44 (15) :3682-90., URL: http://www.ncbi.nlm.nih.gov/pubmed/17524482?ordina, *Desharnais P, Dupéré-Minier G, Hamelin C, Devine P, Bernier J: Involvement of CD45 in DNA fragmentation in apoptosis induced by mitochondrial perturbing agents. Apoptosis. 2007 Dec 19. , URL: http://www.ncbi.nlm.nih.gov/pubmed/18157742?ordina

• Storage Conditions:

  Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.