Products for Research Use Only

TP53 Antibody

CAT: 0013-GTR18129404Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18129404Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
TP53 Antibody
Product Name Alternative
Cellular tumor antigen p53, Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53, TP53, P53
UniProt
P04637
Reactivity
Human
Immunogen
Recombinant human wild-type p53 protein was used as the immunogen for this antibody.
Target
TP53
Clonality
Monoclonal
Clone
BP53-12
Conjugation
Unconjugated
Field of Research
Cancer Biology, Cell Biology, Signal Transduction
Purification
Protein G affinity chromatography
Concentration
0.2 mg/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Flow Cytometry: 0.5-1 ug/million cellsIF: 1-2 μg/mLWB: 0.5-1 μg/mLIHC (FFPE) : 0.5-1 μg/mL for 30 minutes at RT (1) Prediluted format : incubate for 30 min at RT (2) The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Tested Applications
FC, IF, IHC, WB
Host or Source
Mouse
Preservative
PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Isotype
IgG2a

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