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ACTA1 Antibody

CAT: 0013-GTR18129385Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18129385Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
ACTA1 Antibody
Product Name Alternative
Actin, alpha skeletal muscle, Alpha-actin-1, ACTA1, ACTA
UniProt
P68133
Reactivity
Human, Mouse, Rabbit, Rat
Immunogen
SDS extract of human myocardium was used as the immunogen for this Muscle actin HHF35 antibody.
Target
ACTA1
Clonality
Monoclonal
Clone
HHF35
Conjugation
Unconjugated
Field of Research
Musculoskeletal & Connective Tissue Research
Purification
Protein G affinity chromatography
Concentration
0.2 mg/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Western blot: 0.5-1 μg/mLFlow Cytometry: 0.5-1 ug/million cellsIF: 0.5-1 μg/mLIHC (FFPE) : 0.5-1.0 μg/mL for 30 minutes at RT (1) Prediluted format : incubate for 30 min at RT (2) The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Muscle actin HHF35 antibody to be titered up or down for optimal performance.1. No special pretreatment is required for the IHC staining of formalin-fixed, paraffin-embedded tissues.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Tested Applications
FC, IF, IHC, WB
Host or Source
Mouse
Preservative
PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Isotype
IgG1, kappa

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