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AR Antibody

CAT: 0013-GTR18129205Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18129205Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
AR Antibody
Product Name Alternative
Androgen receptor, Dihydrotestosterone receptor, Nuclear receptor subfamily 3 group C member 4, AR, DHTR, NR3C4
UniProt
P10275
Reactivity
Human
Immunogen
Amino acids 302-318 (STEDTAEYSPFKGGYTK) from human AR were used as the immunogen for the anti-Androgen Receptor antibody.
Target
AR
Clonality
Monoclonal
Clone
SPM335
Conjugation
Unconjugated
Field of Research
Cancer Biology, Cell Biology, Signal Transduction
Purification
Protein G affinity chromatography
Concentration
0.2 mg/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 μg/mLImmunohistochemistry (FFPE) : 0.5-1 μg/mL for 30 min at RT (1) Prediluted format : incubate for 30 min at RT (2) Optimal dilution of the anti-Androgen Receptor antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Tested Applications
FC, IF, IHC-P
Host or Source
Mouse
Preservative
PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Isotype
IgG1, kappa

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