Products for Research Use Only

MYH11 Antibody

CAT: 0013-GTR18129174Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18129174Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
MYH11 Antibody
UniProt
P35749
Reactivity
Bovine, Canine, Feline, Gallus, Guinea pig, Human, Porcine, Rabbit, Rat
Immunogen
Human uterus extract was used as the immunogen for the SM-MHC antibody.
Target
MYH11
Clonality
Monoclonal
Clone
SPM201
Conjugation
Unconjugated
Field of Research
Musculoskeletal & Connective Tissue Research
Purification
Protein G affinity chromatography
Concentration
0.2 mg/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 μg/mLImmunohistochemistry (FFPE) : 0.5-1 μg/mL for 30 min at RT (1) Prediluted format: incubate for 30 min at RT (2) The stated application concentrations are suggested starting amounts. Optimal dilution of the SM-MHC antibody should be determined by the researcher.1. Staining of formalin/paraffin tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Tested Applications
FC, IF, IHC-P
Host or Source
Mouse
Preservative
PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Isotype
IgG1, kappa

UniProtKB · P35749

Myosin-11

MYH11_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P35749
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
MYH11
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
D2JYH7, O00396, O94944, P78422, Q3MIV8, Q3MNF0, Q3MNF1
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Molecular function

Actin-bindingCalmodulin-bindingMotor proteinMuscle proteinMyosin

Coding sequence diversity

Alternative splicingChromosomal rearrangement

Disease

Aortic aneurysmDisease variantProto-oncogene

Ligand

ATP-bindingNucleotide-binding

Domain

Coiled coil

PTM

MethylationPhosphoprotein

Cellular component

Thick filament

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