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NKX2-2 Antibody

CAT: 0013-GTR18128628Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18128628Size:100 µg
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Description
NKX2-2 Antibody
Product Name Alternative
NKX2-2 Antibody: NKX2B, NKX2.2, NKX2B, Homeobox protein NK-2 homolog B
UniProt
O95096
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant human protein was used as the immunogen for this antibody.
Target
NKX2-2
Clonality
Monoclonal
Clone
NK2TF-1
Conjugation
Unconjugated
Field of Research
Stem Cell & Developmental Biology
Purification
Protein G affinity chromatography
Concentration
0.2 mg/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
IF: 1-2 μg/mLIHC (FFPE) : 1-2 μg/mL for 30 minutes at RT (1) Prediluted format : incubate for 30 min at RT (2) The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the NKX2.2 antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Tested Applications
IF, IHC
Host or Source
Mouse
Preservative
PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Isotype
IgG2b, kappa

UniProtKB · O95096

Homeobox protein Nkx-2.2

NKX22_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
O95096
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
NKX2-2
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
—
Protein keywords

Molecular function

ActivatorDevelopmental proteinDNA-binding

Domain

Homeobox

Cellular component

Nucleus

Technical term

Proteomics identificationReference proteome

Biological process

TranscriptionTranscription regulation

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