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MYOD1 Antibody

CAT: 0013-GTR18128553Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18128553Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
MYOD1 Antibody
Product Name Alternative
Myoblast determination protein 1, Class C basic helix-loop-helix protein 1, bHLHc1, Myogenic factor 3, Myf-3, MYOD1, BHLHC1, MYF3, MYOD
UniProt
P15172
Reactivity
Gallus, Human, Mouse, Rat
Immunogen
Recombinant mouse protein was used as the immunogen for this anti-MyoD1 antibody. The epitope of this mAb maps between amino acid 180-189.
Target
MYOD1
Clonality
Monoclonal
Clone
SPM427
Conjugation
Unconjugated
Field of Research
Stem Cell & Developmental Biology
Purification
Protein G affinity chromatography
Concentration
0.2 mg/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 μg/mLImmunohistochemistry (FFPE) : 0.5-1 μg/mL for 30 minutes at RT (1) Prediluted format: incubate for 30 min at RT (2) The optimal dilution of the anti-MyoD1 antibody for each application should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Tested Applications
FC, IF, IHC
Host or Source
Mouse
Preservative
PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Isotype
IgG1, kappa

UniProtKB · P15172

Myoblast determination protein 1

MYOD1_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P15172
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
MYOD1
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
O75321
Protein keywords

PTM

AcetylationMethylationPhosphoproteinUbl conjugation

Molecular function

ActivatorDevelopmental proteinDNA-binding

Biological process

DifferentiationMyogenesisTranscriptionTranscription regulation

Disease

Disease variant

Cellular component

Nucleus

Technical term

Proteomics identificationReference proteome

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