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VIM Antibody

CAT: 0013-GTR18129124Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18129124Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
VIM Antibody
Product Name Alternative
Vimentin, VIM
UniProt
P08670
Reactivity
Bovine, Canine, Feline, Gallus, Goat, Human, Porcine
Immunogen
Human recombinant protein was used as the immunogen for this VIM antibody.
Target
VIM
Clonality
Monoclonal
Clone
SPM576
Conjugation
Unconjugated
Field of Research
Cancer Biology, Cell Biology, Neuroscience, Signal Transduction
Purification
Protein G affinity chromatography
Concentration
0.2 mg/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 1-2 μg/mLWestern blot: 0.25-0.5 μg/mLImmunohistochemistry (FFPE) : 0.25-0.5 μg/mL for 30 min at RT (1) Prediluted format: incubate for 30 min at RT (2) The optimal dilution of the VIM antibody for each application should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Tested Applications
FC, IF, IHC, WB
Host or Source
Mouse
Preservative
PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Isotype
IgG1

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