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NKX2-1 Antibody

CAT: 0013-GTR18129406Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18129406Size:100 µg
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Description
NKX2-1 Antibody
Product Name Alternative
NKX2-1 Antibody: BCH, BHC, NK-2, TEBP, TTF1, NKX2A, TITF1, TTF-1, NKX2.1, Homeobox protein NK-2 homolog A
UniProt
P43699
Reactivity
Human, Mouse, Rat
Immunogen
Rat full length recombinant protein was used as the immunogen for this TTF-1 antibody.
Target
NKX2-1
Clonality
Monoclonal
Clone
8G7G3/1
Conjugation
Unconjugated
Purification
Protein G affinity chromatography
Concentration
0.2 mg/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Flow Cytometry: 0.5-1 ug/million cellsIF: 0.5-1 μg/mLIHC (FFPE) : 0.5-1 μg/mL for 30 minutes at RT (1) Prediluted format : incubate for 30 min at RT (2) The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the TTF-1 antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Tested Applications
FC, IF, IHC
Host or Source
Mouse
Preservative
PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Isotype
IgG1, kappa

UniProtKB · P43699

Homeobox protein Nkx-2.1

NKX21_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P43699
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
NKX2-1
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
D3DSA3, O14954, O14955, Q7KZF6, Q9BRJ8
Protein keywords

Molecular function

ActivatorDNA-bindingRepressor

Coding sequence diversity

Alternative splicing

Biological process

Biological rhythmsTranscriptionTranscription regulation

Disease

Congenital hypothyroidismDisease variant

Domain

Homeobox

Cellular component

Nucleus

PTM

Phosphoprotein

Technical term

Proteomics identificationReference proteome

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