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THBS3 Antibody

CAT: 0013-GTR18126552Size: 0.1 mgDry Ice: NoHazardous: No
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CAT#:0013-GTR18126552Size:0.1 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
THBS3 Antibody
Product Name Alternative
THBS3, thrombospondin 3, MGC119564, MGC119565, TSP3, OTTHUMP00000033827
UniProt
P49746
Reactivity
Human
Immunogen
The immunogen for this antibody is: CNDTVPEDFEPFRRQ
Target
THBS3
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Signal Transduction
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave an approx 50kDa band in Human Lung and in lysates of cell line A549 after 0.3μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 104kDa according to NP_009043.1. The 50kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Colon shows endothelial cell staining of a blood vessel. Recommended concentration, 1-2μg/mL.
Prediction Reactivity
Bovine, Canine, Mouse, Porcine
Tested Applications
ELISA, IHC
NCBI Accession Number
NP_009043.1
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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