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NEBL Antibody

CAT: 0013-GTR18126824Size: 0.1 mgDry Ice: NoHazardous: No
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CAT#:0013-GTR18126824Size:0.1 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
NEBL Antibody
Product Name Alternative
NEBL, nebulette, FLJ53769, LNEBL, MGC119746, MGC119747, bA56H7.1, LIM-nebulette, NEBL, actin-binding Z-disc protein, OTTHUMP00000019268, actin-binding Z-disk protein
UniProt
O76041
Immunogen
The immunogen for this antibody is: C-ELQRLKRTQE
Target
NEBL
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Musculoskeletal & Connective Tissue Research
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Preliminary experiments gave an approx 30kDa band in Human Brain (Frontal Cortex) lysates, but not in Human, Mouse and Rat Heart lysates after 1μg/mL antibody staining. This protein has a calculated MW of 31.2kDa according to NP_998734.1. The 30kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?
Prediction Reactivity
Canine, Human, Mouse
Tested Applications
ELISA
NCBI Accession Number
NP_001166955.1, NP_998734.1
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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