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TRIM29 Antibody

CAT: 0013-GTR18126272Size: 0.1 mgDry Ice: NoHazardous: No
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CAT#:0013-GTR18126272Size:0.1 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
TRIM29 Antibody
Product Name Alternative
TRIM29, tripartite motif-containing 29, ATDC, FLJ36085, ataxia-telangiectasia group D-associated protein, tripartite motif protein TRIM29
UniProt
Q14134
Reactivity
Human
Immunogen
The immunogen for this antibody is: C-EKDRIKSFTTNE
Target
TRIM29
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 37kDa band observed in Human Peripheral Blood Mononucleocytes (PBM) and in lysates of cell lines HeLa. An additional 70kDa band was seen in some PBM lysates (calculated MW of 37.3kDa according to NP_001317311.1 (isoform 2) and 65.8kDa according to NP_036233.2 (isoform 1) . Recommended concentration: 0.1-1μg/mL. Primary incubation 1 hour at room tempetature.Immunohistochemistry:In paraffin embedded Human Skin shows staining of subnuclear structures and cytoplasm in keratinocytes. Recommended concentration: 3.75μg/mL.Immunofluorescence: Strong expression of the protein seen in the Cytoplasm and Intermediate filaments of A431 cells. Strong expression of the protein seen in the Nucleus of U2OS cells. Recommended concentration: 10μg/mL. Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10μg/mL.
Prediction Reactivity
Bovine, Mouse, Rat
Tested Applications
ELISA, FC, IF, IHC, WB
NCBI Accession Number
NP_001317311.1, NP_036233.2
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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