Products for Research Use Only

PLA2G4A Antibody

CAT: 0013-GTR18126172Size: 0.1 mgDry Ice: NoHazardous: No
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CAT#:0013-GTR18126172Size:0.1 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
PLA2G4A Antibody
Product Name Alternative
PLA2G4A, phospholipase A2, group IVA (cytosolic, calcium-dependent), MGC126350, PLA2G4, cPLA2-alpha, calcium-dependent phospholipid-binding protein, cytosolic phospholipase A2, group IVA, lysophospholipase, phosphatidylcholine 2-acylhydrolase
UniProt
P47712
Reactivity
Human
Immunogen
The immunogen for this antibody is: C-QEKTFRQQRKEHIR
Target
PLA2G4A
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Metabolism Research
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 80-85kDa band observed in lysates of cell line A549 (calculated MW of 85.2kDa according to NP_077734.1) . Preliminary experiments also showed an 85- 90kDa band in lysates of cell lines A431, HeLa and NIH-3T3 Recommended concentration:0.3-1μg/mL. Primary incubation 1 hour at room temperature.Preliminary testing was unsuccessful on Rat for this particular batch.Immunohistochemistry:Paraffin embedded Human Uterus. Recommended concentration: 3.75μg/mL.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A549 cells. Recommended concentration: 10μg/mL. Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10μg/mL.
Prediction Reactivity
Mouse, Rat
Tested Applications
ELISA, FC, IF, IHC, WB
NCBI Accession Number
NP_077734.1
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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