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MKRN1 Antibody

CAT: 0013-GTR18125926Size: 0.1 mgDry Ice: NoHazardous: No
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CAT#:0013-GTR18125926Size:0.1 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
MKRN1 Antibody
Product Name Alternative
MKRN1, makorin ring finger protein 1, FLJ21334, RNF61, E3 ubiquitin-protein ligase makorin-1, OTTHUMP00000212121, OTTHUMP00000212124, RING finger protein 61
UniProt
Q9UHC7
Reactivity
Human, Mouse
Immunogen
The immunogen for this antibody is: C-RYEHSKPLKQEEAT
Target
MKRN1
Clonality
Polyclonal
Conjugation
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 50kDa band observed in fetal Mouse Brain lysates and 35kDa plus fainter 50kDa bands in adult Mouse Brain lysates (calculated MW of 52.9kDa according to NP_061280.2) . The observed 35kDa band corresponds to the calculated MW of human isoform 2 (35.2kDa according to NP_001138597.1), but this size isoform has not yet been confirmed in Mouse. Recommended concentration: 1-3μg/mL. In transfected HEK293 transiently expressing full-length Human MKRN1 (myc and DYKDDDDK tagged), a band of approx. 60kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDDK tag antibody. Recommended concentration, 0.3- 1μg/mL.Purchase the +ve control! The transfected lysate used to QC this product is available for sale. Please email [email protected] to request datasheet, pricing and delivery information.
Prediction Reactivity
Bovine, Canine, Porcine
Tested Applications
ELISA, WB
NCBI Accession Number
NP_038474.2, NP_001138597.1
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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