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HDAC1 Antibody

CAT: 0013-GTR18125800Size: 0.1 mgDry Ice: NoHazardous: No
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CAT#:0013-GTR18125800Size:0.1 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
HDAC1 Antibody
Product Name Alternative
HDAC1, histone deacetylase 1, DKFZp686H12203, GON-10, HD1, RPD3, RPD3L1 , reduced potassium dependency, yeast homolog-like 1
UniProt
Q13547
Reactivity
Human, Mouse
Immunogen
The immunogen for this antibody is: C-KPEAKGVKEEVK
Target
HDAC1
Clonality
Polyclonal
Conjugation
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Molecular Weight
Appox 55 kDa
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Appox 55kDa band observed in Human Duodenum and Lymph Node lysates and approx 70kDa band observed in lysates of cell lines HeLa and NIH3T3 (calculated MW of 55.1kDa according to Human NP_004955.2 and Mouse NP_032254.1) . The observed 70kDa band represents the sumoylated protein according to David et al, J Biol Chem. 2002 Jun 28;277 (26) :23658-63; PMID: 11960997. Recommended concentration: 0.1-2μg/mL.Immunofluorescence: Strong expression of the protein seen in the nucleus of NIH3T3 cells. Recommended concentration: 10μg/mL Chromatin Immunoprecipitation: A 3.5-fold increased presence of H3 onto the Human P21 locus observed when comparing the precipitations using EB07186 with non-specific goat IgG to bring down HDAC1 in a chromatin lysate from MCF7 cells.
Prediction Reactivity
Canine, Rat
Tested Applications
ChIP, ELISA, IF, WB
NCBI Accession Number
NP_004955.2
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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