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Makorin Antibody

CAT: 0013-GTR18125671Size: 0.1 mgDry Ice: NoHazardous: No
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CAT#:0013-GTR18125671Size:0.1 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Makorin Antibody
Product Name Alternative
MKRN1, makorin, ring finger protein 1, HGNC:7112, RNF61, makorin ring finger protein 1, FLJ21334
UniProt
Q9UHC7
Reactivity
Human
Immunogen
The immunogen for this antibody is: C-VTKEESEKEQTAVET
Target
Makorin
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Cell Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:In transfected HEK293 transiently expressing full-length Human MKRN1 (myc and DYKDDDDK tagged), a band of approx. 60kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDDK tag antibody. Recommended concentration, 1-2μg/mL.
Prediction Reactivity
Canine
Tested Applications
ELISA, WB
NCBI Accession Number
NP_038474.1, NP_001138597.1
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

UniProtKB · Q9UHC7

E3 ubiquitin-protein ligase makorin-1

MKRN1_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q9UHC7
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
MKRN1
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
2.3.2.27
Processing
—
Secondary accessions
A4D1T7, B3KXB4, Q256Y7, Q59G11, Q6GSF1, Q9H0G0, Q9UEZ7, Q9UHW2
Protein keywords

Coding sequence diversity

Alternative splicing

Ligand

Metal-bindingZinc

Technical term

Proteomics identificationReference proteome

Domain

RepeatZinc-finger

Molecular function

Transferase

PTM

Ubl conjugation

Biological process

Ubl conjugation pathway

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