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IRAK4 Antibody

CAT: 0013-GTR18126324Size: 0.1 mgDry Ice: NoHazardous: No
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CAT#:0013-GTR18126324Size:0.1 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
IRAK4 Antibody
Product Name Alternative
IRAK4, REN64, NY-REN-64, interleukin-1 receptor-associated kinase 4, IRAK-4 mutated form 1, interleukin-1 receptor associated kinase 4, interleukin-1 receptor associated kinase 4 mutant form 1, IPD1
UniProt
Q9NWZ3
Reactivity
Human
Immunogen
The immunogen for this antibody is: NKPITPSTYVRC
Target
IRAK4
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 50kDa band observed in lysates of cell line HeLa and approx 55kDa in lysates of cell line Jurkat (calculated MW of 51.5kDa according to NP_057207.1) . This molecular weight is routinely observed by other sources. Recommended concentration: 0.5-2μg/ml. Primary incubation 1 hour at room temperature. IHC: Paraffin embedded Human Heart and Human Liver. Recommended concentration: 3.75μg/ml. Immunofluorescence: Strong expression of the protein seen in the cytoplasm and microtubules of HeLa cells. Recommended concentration: 10μg/ml. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10μg/mL.
Prediction Reactivity
Mouse
Tested Applications
ELISA, FC, ICC, IHC, WB
NCBI Accession Number
NP_001107654.1, NP_057207.2
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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