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ACHE Antibody

CAT: 0013-GTR18125364Size: 0.1 mgDry Ice: NoHazardous: No
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CAT#:0013-GTR18125364Size:0.1 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
ACHE Antibody
Product Name Alternative
ACHE, YT, acetylcholinesterase (YT blood group), ARACHE, N-ACHE, OTTHUMP00000211347, OTTHUMP00000211349, OTTHUMP00000211356, acetylcholinesterase, apoptosis-related acetylcholinesterase
UniProt
P22303
Reactivity
Human, Rat
Immunogen
The immunogen for this antibody is: QFDHYSKQDRCSDL
Target
ACHE
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Signal Transduction
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 70kDa band observed in HepG2 and Jurkat cell lysates and approx. 65-70kDa in Daudi and HeLa cell lysates (calculated MW of 67.8kDa according to NP_000656.1) . Recommended concentration: 0.3-1μg/ml. Primary incubation 1 hour at room temperature.Immunofluorescence: Strong expression of the protein seen in the cytoplasm and nuclei of U2OS cells. Recommended concentration: 10μg/mL. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10μg/mL.
Prediction Reactivity
Mouse
Tested Applications
ELISA, FC, IF, WB
NCBI Accession Number
NP_001269378.1, NP_000656.1
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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