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Robo3.1 Antibody

CAT: 0013-GTR18125272Size: 0.1 mgDry Ice: NoHazardous: No
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CAT#:0013-GTR18125272Size:0.1 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Robo3.1 Antibody
Product Name Alternative
Robo3, roundabout homolog 3 (Drosophila), Rbig1, Rig-1, Rig1, Robo3a, Robo3b, retinoblastoma inhibiting gene 1, retinoblastoma-inhibiting gene 1 protein, roundabout homolog 3
UniProt
Q9Z2I4
Immunogen
The immunogen for this antibody is: C-PYGRPSFLSHGQGT
Target
Robo3.1
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Neuroscience
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Preliminary experiments gave bands at approx 100kDa and 80kDa in Mouse fetal Brain lysates after 1μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 150kDa according to NP_001158239. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands) . We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Prediction Reactivity
Mouse
Tested Applications
ELISA
NCBI Accession Number
NP_001158239.1
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

UniProtKB · Q9Z2I4

Roundabout homolog 3

ROBO3_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
Q9Z2I4
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Robo3
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
A0A1L1SU85, D3Z4M6
Protein keywords

Coding sequence diversity

Alternative splicing

Biological process

ChemotaxisDifferentiationNeurogenesis

Molecular function

Developmental proteinReceptor

PTM

Disulfide bondGlycoproteinPhosphoprotein

Domain

Immunoglobulin domainRepeatSignalTransmembraneTransmembrane helix

Cellular component

Membrane

Technical term

Reference proteome

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