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FTCD Antibody

CAT: 0013-GTR18125077Size: 0.1 mgDry Ice: NoHazardous: No
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CAT#:0013-GTR18125077Size:0.1 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
FTCD Antibody
Product Name Alternative
FTCD, LCHC1, formiminotransferase cyclodeaminase, formimidoyltransferase cyclodeaminase, LC1 autoantigen
UniProt
O95954
Reactivity
Human, Mouse, Porcine
Immunogen
The immunogen for this antibody is: CLREQGRGKDQPGRL
Target
FTCD
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Metabolism Research
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 60kDa band observed in Human Liver lysates and approx. 55-60kDa in Mouse and Pig Liver lysates, and also in preliminary testing of Rat Liver, Human Kidney and U2OS lysates (calculated MW of 58.9kDa according to Human NP_006648.1, Mouse NP_543121.1 and Pig NP_999440.1) . Recommended concentration: 0.03-0.3μg/mL. Primary incubation 1 hour at room temperature.Immunohistochemistry:In paraffin embedded Human Liver shows distinct staining of membranes and cytoplasm in a selection of hepatocytes. Recommended concentration: 3-5μg/mL.Immunofluorescence: Strong expression of the protein seen in the cytoplasm, membranes and plasma membranes of HeLa and in the plasma membranes of HepG2 cells. Recommended concentration: 10μg/mL. Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10μg/mL.
Prediction Reactivity
Bovine, Rat
Tested Applications
ELISA, FC, IF, IHC, WB
NCBI Accession Number
NP_996848.1, NP_001307341.1, NP_006648.1
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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