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GAPDH Antibody

CAT: 0013-GTR18125073Size: 0.1 mgDry Ice: NoHazardous: No
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CAT#:0013-GTR18125073Size:0.1 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
GAPDH Antibody
Product Name Alternative
GAPDH, glyceraldehyde-3-phosphate dehydrogenase, HGNC:4141, G3PD, GAPD, MGC88685, OTTHUMP00000174431, OTTHUMP00000174432, aging-associated gene 9 protein, glyceraldehyde 3-phosphate dehydrogenase
UniProt
P04406
Reactivity
Human, Mouse, Rat
Immunogen
The immunogen for this antibody is: C-GVNHEKYDNSLK
Target
GAPDH
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Metabolism Research
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 37kDa band observed in Human Liver, Testis and Tonsil, and in Mouse Liver and Rat Heart lysates, and in cell lysates of HeLa and NIH3T3 (calculated MW of 36.1kDa according to Human NP_002037.2, Mouse NP_032110.1, and Rat NP_058704.1) . Recommended concentration: 0.03-0.1μg/mL. Primary incubation 1 hour at room temperature. This product has been successfully used in WB on Human: PMID: 29953622 and PMID: 29158223.Immunohistochemistry:In paraffin embedded Human Liver shows textured cytoplasm staining in hepatocytes. Recommended concentration: 2μg/mL.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U251 and HeLa cells. Recommended concentration: 5-10μg/mL.
Prediction Reactivity
Canine
Tested Applications
ELISA, IF, IHC, WB
NCBI Accession Number
NP_001243728.1, NP_002037.2
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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