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GRB2 Antibody

CAT: 0013-GTR18125067Size: 0.1 mgDry Ice: NoHazardous: No
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CAT#:0013-GTR18125067Size:0.1 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
GRB2 Antibody
Product Name Alternative
GRB2, growth factor receptor-bound protein 2, ASH, ASH protein, EGFRBP-GRB2, Grb3-3, MST084, MSTP084, HT027, abundant SRC homology, epidermal growth factor receptor-binding protein GRB2, growth factor receptor-bound protein 3, NCKAP2, OTTHUMP00000166096, OTTHUMP00000166097, OTTHUMP00000166098, abundant SRC homology
UniProt
P62993
Reactivity
Human, Mouse, Porcine, Rat
Immunogen
The immunogen for this antibody is: C-PRNYVTPVNRNV
Target
GRB2
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Signal Transduction
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 26kDa band observed in lysates of cell line MOLT4, of Human Thymus and of Human, Mouse, Rat, Pig Brain and Spleen lysates (calculated MW of 25.2kDa according to Human NP_002077.1 and according to Rat NP_110473.2) . Recommended for use at 0.05-0.15μg/mL.Immunohistochemistry:In paraffin embedded Human Lymph Node shows nuclear staining in some cells inside the germinal centre. Recommended concentration: 1-2μg/mL.Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
Prediction Reactivity
Bovine, Canine
Tested Applications
ELISA, IHC, WB
NCBI Accession Number
NP_002077.1, NP_987102.1
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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