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VHL Antibody

CAT: 0013-GTR18124965Size: 0.1 mgDry Ice: NoHazardous: No
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CAT#:0013-GTR18124965Size:0.1 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
VHL Antibody
Product Name Alternative
VHL, von Hippel-Lindau tumor suppressor, HRCA1, RCA1, VHL1 , elongin binding protein, von Hippel-Lindau syndrome
UniProt
P40337
Reactivity
Human, Mouse, Rat
Immunogen
The immunogen for this antibody is: C-RSLVKPENYRRLD
Target
VHL
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Cell Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 17kDa band observed in Human Ovary and Testis lysates and also in lysates of Mouse and Rat Testis and Brain and of all human and mouse cell lines tested (calculated MW of 19.7kDa according to NP_937799.1) . Recommended concentration: 0.01-0.03μg/mL. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Kidney. Recommended concentration: 5μg/mL.Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant VHL captured by a rabbit antibody. Recommended reporter concentration: 1.5-3μg/mL
Prediction Reactivity
Canine
Tested Applications
EIA, ELISA, IHC, WB
NCBI Accession Number
NP_937799.1, NP_000542.1
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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