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ABCB10 Antibody

CAT: 0013-GTR18124958Size: 0.1 mgDry Ice: NoHazardous: No
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CAT#:0013-GTR18124958Size:0.1 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
ABCB10 Antibody
Product Name Alternative
ABCB10, ATP-binding cassette, sub-family B (MDR/TAP), member 10, EST20237, M-ABC2, MTABC2, ATP-binding cassette, sub-family B, member 10, ABC-me
UniProt
Q9NRK6
Immunogen
The immunogen for this antibody is: C-KPNGIYRKLMNKQ
Target
ABCB10
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Cancer Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Preliminary experiments gave bands at approx. 90kDa and 50kDa in Human Bone Marrow lysates after 0.05μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 79.1kDa according to NP_036221.2 and given the expected mature size of ~65kDa according to Graf et al. (PMID: 15215243) . Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands) . We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Prediction Reactivity
Human, Mouse, Rat
Tested Applications
ELISA
NCBI Accession Number
NP_036221.2
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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