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CARD6 Antibody

CAT: 0013-GTR18124785Size: 0.1 mgDry Ice: NoHazardous: No
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CAT#:0013-GTR18124785Size:0.1 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
CARD6 Antibody
Product Name Alternative
CARD6, caspase recruitment domain family, member 6, caspase recruitment domain protein 6, CINCIN1, CARD-containing inhibitor of Nod1 and Cardiak-induced NF-kB activation
UniProt
Q9BX69
Reactivity
Human
Immunogen
The immunogen for this antibody is: ATESTPSEIIERER-C
Target
CARD6
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Cell Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx. 45-50kDa and 25-30kDa bands observed in A549, Human Heart and Human Skeletal Muscle lysates at 1ug of primary. Please note that the bands we observe are different from the predicted size of approx. 119kDa according to NP_115976. Currently we cannot find an explanation for this in the literature. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Pancreas shows variable staining of cytoplasm in acinar cells. Recommended concentration, 5-10μg/mL.
Tested Applications
ELISA, IHC
NCBI Accession Number
NP_115976.2
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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