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DCTN1 Antibody

CAT: 0013-GTR18124655Size: 0.1 mgDry Ice: NoHazardous: No
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CAT#:0013-GTR18124655Size:0.1 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
DCTN1 Antibody
Product Name Alternative
DCTN1, dynactin 1 (p150, glued homolog, Drosophila), P135, DP-150, DAP-150, p150-glued, p150, Glued (Drosophila) homolog, 150 kDa dynein-associated polypeptide, dynactin 1 (p150, Glued (Drosophila) homolog), HMN7B, OTTHUMP00000202491, dynactin 1, p150, Glued homolog
UniProt
Q14203
Reactivity
Human, Mouse
Immunogen
The immunogen for this antibody is: C-QEQLHQLHSRLIS
Target
DCTN1
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Neuroscience
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 150kDa band observed in Human Cerebellum lysates (calculated MW of 142kDa according to NP_004073.2) . Additional bands between approx. 40-70kDa were consistently observed, and all were successfully blocked by incubation with the immunizing peptide. Recommended concentration 1-2μg/mL. Primary incubation 1 hour at room temperature. Preliminary testing was unsuccessful on Rat Brain and Testis for this particular batch.Immunohistochemistry:In paraffin embedded Human Cerebellum shows pixulate staining of cytoplasm in neuronal cells. Recommended concentration: 2-4μg/mL. A customer found positive results in IHC on Human HeLa cells.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa and U2OS cells. Recommended concentration: 10μg/mL. Immunocytochemsitry: A customer found positive results in ICC on Mouse embryonic fibroblasts (MEF cells) . Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10μg/mL.
Tested Applications
ELISA, FC, ICC, IF, IHC, WB
NCBI Accession Number
NP_075408.1, NP_001128513.1, NP_001177766.1, NP_001177765.1, NP_004073.2, NP_001128512.1
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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