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PPP1R15A Antibody

CAT: 0013-GTR18125401Size: 0.1 mgDry Ice: NoHazardous: No
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CAT#:0013-GTR18125401Size:0.1 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
PPP1R15A Antibody
Product Name Alternative
PPP1R15A, GADD34, protein phosphatase 1, regulatory (inhibitor) subunit 15A, growth arrest and DNA-damage-inducible 34, protein phosphatase 1, regulatory subunit 15A
UniProt
O75807
Reactivity
Human
Immunogen
The immunogen for this antibody is: C-AAALDLSGRRG
Target
PPP1R15A
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Cell Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 75kDa band observed in lysates of cell line HepG2 (calculated MW of 73.4kDa according to NP_055145.2) . Recommended concentration: 0.3-1μg/mL. Primary incubation 1 hour at room temperature. Negative Control: KLY (Kelly Human neuroblastoma) cell lysate.Immunohistochemistry:In paraffin embedded Human Liver shows cytoplasmic staining consistent with ER in the hepatocytes. Recommended concentration: 2-4μg/mL.Immunofluorescence: Strong expression of the protein seen in the nuclei of HeLa and HepG2 cells. Recommended concentration: 10μg/mL. Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10μg/mL.
Tested Applications
ELISA, FC, IF, IHC, WB
NCBI Accession Number
NP_055145.3
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

UniProtKB · O75807

Protein phosphatase 1 regulatory subunit 15A

PR15A_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
O75807
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
PPP1R15A
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
B4DKQ3, Q6IA96, Q9NVU6
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Biological process

ApoptosisStress responseTranslation regulation

Cellular component

Endoplasmic reticulumMembraneMitochondrionMitochondrion outer membrane

PTM

PhosphoproteinUbl conjugation

Domain

Repeat

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