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GRIK1 Antibody

CAT: 0013-GTR18125168Size: 0.1 mgDry Ice: NoHazardous: No
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CAT#:0013-GTR18125168Size:0.1 mg
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Description
GRIK1 Antibody
Product Name Alternative
GLUR5, GRIK1, glutamate receptor, ionotropic, kainate 1, EAA3 , EEA3 , GLR5 , excitatory amino acid receptor 3
UniProt
P39086
Reactivity
Human
Immunogen
The immunogen for this antibody is: QCKQTHPTNSTS
Target
GRIK1
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Neuroscience
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 150kDa band observed in Human Brain (Cerebellum and Cerebral Cortex) lysates (calculated MW of 104kDa according to NP_000821.1) . The observed molecular weight corresponds to earlier findings in literature with different antibodies (Christensen et al , J Neurosci. 2004 Oct 13;24 (41) :8986-93.; PMID: 15483117) . Recommended concentration: 1-3μg/mL. Primary incubation was 1 hour. An additional very strong band of unknown identity was also consistently observed at 60kDa. This band was successfully blocked by incubation with the immunising peptide.Immunohistochemistry:Paraffin embedded Human Brain (Cortex) . Recommended concentration: 3.75μg/mL.
Prediction Reactivity
Mouse, Rat
Tested Applications
ELISA, IHC, WB
NCBI Accession Number
NP_000821.1, NP_783300.1
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

UniProtKB · P39086

Glutamate receptor ionotropic, kainate 1

GRIK1_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P39086
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
GRIK1
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
GluK1
EC number
—
Processing
Precursor
Secondary accessions
Q13001, Q86SU9
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Coding sequence diversity

Alternative splicingRNA editing

Cellular component

Cell membraneMembranePostsynaptic cell membraneSynapse

PTM

Disulfide bondGlycoproteinPhosphoprotein

Molecular function

Ion channelLigand-gated ion channelReceptor

Biological process

Ion transportTransport

Domain

SignalTransmembraneTransmembrane helix

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