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HMGA1 Antibody

CAT: 0013-GTR18124613Size: 0.1 mgDry Ice: NoHazardous: No
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CAT#:0013-GTR18124613Size:0.1 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
HMGA1 Antibody
Product Name Alternative
HMGA1, high mobility group AT-hook 1 , HGNC:5010, HMG-R, HMGIY, MGC12816, MGC4242, MGC4854 , OTTHUMP00000039618, high-mobility group (nonhistone chromosomal) protein isoforms I and Y, nonhistone chromosomal high-mobility group protein HMG-I/HMG-Y, HMGA1A, OTTHUMP00000016223, OTTHUMP00000016224
UniProt
P17096
Reactivity
Human
Immunogen
The immunogen for this antibody is: C-LASKQEKDGTEK
Target
HMGA1
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Cancer Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave an approx 50kDa band in Human Testis and Ovary lysates after 0.3μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 11.7kDa according to isoform a (also called HMG-I; NP_665906.1; NP_665908.1; NP_665911.1) and 10.7kDa according to isoform b ((also called HMG-Y; NP_002122.1; NP_665909.1; NP_665910.1; NP_665912.1) . The 50kDa band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:Paraffin embedded Human Placenta and Small Intestine. Recommended concentration: 4μg/mL.
Prediction Reactivity
Bovine, Canine, Mouse, Porcine, Rat
Tested Applications
ELISA, IHC
NCBI Accession Number
NP_665910.1, NP_665909.1, NP_665912.1, NP_665906.1, NP_665908.1, NP_002122.1
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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