Human DPPIV / CD26 Protein, Fc Tag (active enzyme)

Human DPPIV / CD26 Protein, Fc Tag (active enzyme)

• Background: Dipeptidyl peptidase-IV (DPPIV) is also known as adenosine deaminase complexing protein 2, DPPIV or CD26 is antigenic enzyme expressed on the surface of most cell types and is associated with immune regulation, signal transduction and apoptosis. It is an intrinsic membrane glycoprotein and a serine exopeptidase that cleaves X-proline dipeptides from the N-terminus of polypeptides. The substrates of DPPIV are proline (or alanine)-containing peptides and include growth factors, chemokines, neuropeptides, and vasoactive peptides. DPPIV plays a major role in glucose metabolism. It is responsible for the degradation of incretins such as GLP-1. DPPIV plays an important role in tumor biology, and is useful as a marker for various cancers, with its levels either on the cell surface or in the serum increased in some neoplasms and decreased in others. DPPIV also binds the enzyme adenosine deaminase specifically and with high affinity. The significance of this interaction has yet to be established.
• Description: Human DPPIV, Fc Tag (DP4-H5266) is expressed from human 293 cells (HEK293). It contains AA Asn 29 - Pro 766 (Accession # NP_001926.2).
• Specifications: This protein carries a human IgG1 Fc tag at the N-terminus. The protein has a calculated MW of 112.3 kDa. The protein migrates as 90-116 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expression Region: Asn 29 - Pro 766
• Host: HEK 293
• Target: DPPIV
• Conjugation: Unconjugated
• Tag: N-Fc
• Source: Human
• Stability: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Endotoxin: 1.0 EU per μg
• Purity: 95%
• Bioactivity: Immobilized Human DPPIV, Fc Tag (Cat. No. DP4-H5266) at 2 μg/mL (100 μL/well) can bind MERS S1 protein, His Tag (Cat. No. S1N-M52H5) with a linear range of 0.039-0.156 μg/mL (Routinely tested).
• Format: Powder
• Buffer: 50 mM Tris, 100 mM Glycine, pH7.5
• Additives: Trehalose
• Molecular Weight: 112.3 kDa
• Shipping Conditions: Room Temperature
• Storage Conditions: -20°C