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OGT Antibody

CAT: 0013-GTR18124336Size: 0.1 mgDry Ice: NoHazardous: No
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CAT#:0013-GTR18124336Size:0.1 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
OGT Antibody
Product Name Alternative
OGT, O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase), FLJ23071, HRNT1, MGC22921, O-GLCNAC , O-GlcNAc transferase p110 subunit O-linked GlcNAc transferase, uridinediphospho-N-acetylglucosamine:polypeptide beta-N-acetylglucosaminyl transferase
UniProt
O15294
Reactivity
Human, Rat
Immunogen
The immunogen for this antibody is: C-YEHPKDLKLSDGR
Target
OGT
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Epigenetics & Chromatin
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 110kDa band observed in Rat Pancreas lysates calculated MW of 116kDa according to NP_858058.2) . An additional band of unknown identity was also consistently observed at 60kDa. This band was successfully blocked by incubation with the immunising peptide. Recommended concentration: 0.05-0.2μg/mL. Primary incubation 1 hour at room temperature. Preliminary testing was unsuccessful on Mouse Brain for this particular batch.Immunohistochemistry:Paraffin embedded Human Brain (Cortex) . Recommended concentration: 5μg/mL.Immunofluorescence: Strong expression of the protein seen in the nucleus of HeLa, U2OS and Glioblastoma U251 cells. Recommended concentration: 10μg/mL. Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10μg/mL.
Prediction Reactivity
Bovine, Canine, Mouse
Tested Applications
ELISA, FC, IF, IHC, WB
NCBI Accession Number
NP_858058.1, NP_858059.1
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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