Goat Serum
CAT:
246-IHR-8136
Size:
20 mL
Price:
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- Availability: 24/48H Stock Items & 2 to 6 Weeks non Stock Items.
- Dry Ice Shipment: No

Goat Serum
- Description: This serum is used as blocking agents at 5% in buffer with all Immunoassays, to reduce non-specific background and conserved sequence. This serum can also be used as a negative control in immunoassays with proper dilution.
- Applications: IHC, ICC
- Assay Principle: For Immunohistochemistry (IHC) and Immunocytochemistry (ICC) 1. If tissues are used, deparafinize and hydrate tissue sections through xylene or other clearing agents and graded alcohols.(For frozen sections or cell smears; use unfixed, acetone fixed or appropriate fixative for the antigen in question; for cell smears it may be necessary to permealize the cell by detergent, please refer to antibody protocol. 2. Wash 2-3 with distilled or deionized water. 3. Incubate sections/cell smear in Endoblocker (#1) for 5-10 minutes at room temperature or 37°C. Note: If antigen retriever is required it can be applied after this stage. 4. Wash slide with PBS or Tris saline (with 0.02-0.05% nonionic detergent, Triton X100, Tween 20 or NP-40) or washing buffer (Immuno Automation buffer IBSC cat # AR-6561) 3-5X. 5. Incubate sections/ cell smear in Protein blocking solution (diluted animal serum), for 10 minutes. at RT or 37ºC 6. Wash slide with PBS 1X. 7. Follow instructions for IHC/ICC. The diluted serums are also used as blocking agents in all immunoassays.
- Shipping Conditions: Blue Ice
- Storage Conditions: 4°C