OSBPL9 Antibody

OSBPL9 Antibody

OSBPL9, ORP9, FLJ12492, FLJ14629, FLJ14801, FLJ32055, FLJ34384, MGC15035, oxysterol binding protein-like 9, OSBP-related protein 9, oxysterol-binding protein-related protein 9

Q96SU4

Human

The immunogen for this antibody is: C-EPLLKRLGAAKH

OSBPL9

Unconjugated

Signal Transduction

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

500 ug/mL

Liquid

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

For research use only.

Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave bands at approx 150kDa and 19kDa in lysates of Human Brain, and Testis and 55kDa in lysates of Human Placenta and Testes after 0.5 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the different calculated sizes of the six reported isoforms (a: 62.5kDa for NP_683702.1 and NP_683703.1; b: 63.9kDa for NP_683704.1; c: 70.2kDa for NP_683705.1; d: 81.8kDa for NP_683706.1; e: 83.2 kDa for NP_078862.2; f: 84.3kDa for NP_683707.1) . All detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands) . We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Lung shows staining of alveolar macrophages. . Recommended concentration, 2-4μg/mL.

Bovine, Canine, Mouse, Porcine, Rat

ELISA, IHC

NP_683704.2, NP_683705.1, NP_078862.4, NP_683702.1, NP_683706.3, NP_683707.3

Goat